# Haemocytometer Calculation

This is a simple calculation to observe large microbial cells (e.g. yeast) in a haemocytometer chamber.
Here you have to estimate the number of cells that would be present in a given volume of the original culture and you can check your answer

This is a simple calculation to observe large microbial cells (e.g. yeast) in a haemocytometer chamber.
Here you have to estimate the number of cells that would be present in a given volume of the original culture and you can check your answer

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#### Formula

Hemocytometer Calculations Suppose that 20 cells are counted in the cytometer, area 1 mm2 and depth 0.02 mm.Calculate the total number of cells in the original volume of 5 cm3 of broth.
* Vol of chamber = 1 mm x 1 mm x 0.02 mm = 0.02 mm3
* 1 cm3 = 1000 mm3 ? vol = 2 x 10^{5} cm3
* ? concentration = 20/(2 x 10^{5}) = 106 cells per cm3
* ? number in 5 cm3 = 5 x 106 cells
This online microbiological calculator helps you dynamically to calculate the Haemocytometer easily.

**Note :**This statistics calculator is presented for your own personal use and is to be used as a guide only. Medical and other decisions should NOT be based on the results of this calculator. Although this calculator has been tested, we cannot guarantee the accuracy of its calculations or results.