Here is a simple online calculator tool to perform matrix subtraction between two 4 x 4 matrices A and B. A 4x4 matrix is an array of numbers with 4 columns and 4 rows. Enter the values for both matrices in the fields given below and click calculate to get the result. Addition and subtraction of matrices are used in geometry, physics and computer graphics applications. The below 4x4 matrix subtraction tool helps you to make your calculations easy and effortless.